Microscopy Matters

Insights from the Advanced Imaging Center at Janelia

January 14, 2015
by John Heddleston, Ph.D.
Comments Off on Objective Orientation in the Lattice Light Sheet Microscope

Objective Orientation in the Lattice Light Sheet Microscope

How is orientation of the Lattice Light Sheet Microscope objectives different? As mentioned in an earlier post, the lattice light sheet miscroscope (LLSM) requires special consideration when preparing and mounting samples for imaging. This is due to the size of … Continue reading

December 11, 2014
by Lin Shao, Ph.D.
Comments Off on Structured-Illumination Microscopy: What types of samples are not suitable and why

Structured-Illumination Microscopy: What types of samples are not suitable and why

Structured-Illumination Microscopy: What types of sample are not suitable and why Needless to say that, just like in any type of microscopy, low signal samples in general do not work well for SIM. Besides that, there are other factors unrelated … Continue reading

November 17, 2014
by John Heddleston, Ph.D.
Comments Off on Lattice Light Sheet Microscopy Sample Prep

Lattice Light Sheet Microscopy Sample Prep

The lattice light sheet microscope (LLSM) is a powerful optical tool for observing rapidly-occurring processes in live samples. This technique offers multiple advantages over more common optical microscopy methods, like spinning-disk confocal, with improved optical sectioning, reduced photobleaching, and minimized … Continue reading