Microscopy Matters

Insights from the Advanced Imaging Center at Janelia

February 12, 2016
by Jesse Aaron, Ph.D.
Comments Off on Multi-Color Single Molecule Localization Microscopy (SMLM)

Multi-Color Single Molecule Localization Microscopy (SMLM)

Introduction In a previous blog post, we discussed probe selection for single molecule localization microscopy (SMLM) techniques such as PALM and STORM. This class of techniques is powerful in that it can achieve some of the highest spatial resolutions (in … Continue reading

November 6, 2014
by Teng-Leong Chew, Ph.D.
Comments Off on Some Thoughts on Fluorescence Recovery Analysis

Some Thoughts on Fluorescence Recovery Analysis

The inherent tendency of fluorescent molecules to photobleach has been turned into a very powerful tool for the study of molecular trafficking. By targeting a region of interest for laser-mediated photobleaching, biologists can study how fast the molecules go in … Continue reading